Your machine is in inches or mm?
What pitch leadscrews are you using?
When working on a machine you have never tested before, it's good to start low on acceleration and max speed. Slowly increase acceleration with testing after each change. You can test it by placing a DTI and measure if it returns to 0 after a move (make sure that you don't include backlash while doing this). When you start losing steps, you should pull back a bit.
It needs some trial and error, but it's easy enough to figure it out.