I have a similar setup. I am using a xylotex controller and steppers.
My startup routine is this:
1)Boot up the computer
2)Once booted I turn on the controller
3)Start up Mach
4)Mach starts in " Emergengy mode active", basically in a system pause / Emergency stop mode. This may be your issue. You will see a red rectangular bar in the lower left corner of the screen with a "Reset" button below it. You will need to push that to deactivate the Emergency stop.
This should allow your machine to move. I say should because this takes into account that Mach has been properly configured to work with the machine.
Even then it should work even with the motors out of tune etc etc.
At the very least try that reset and see if you get motion.
Let me know if that works to get things going