Most likely not if they follow a normal format, Mach3 is very forgiving, as to how a program is formatted, you can almost through anything at it and it will run
Post a program here, usually if it will load into Mach3, it will run, which you can try that with the Mach3 you have loaded on your computer, it does not need to be connected to anything to do this, you can run it as well, if all is good you will see it working on the Mach3 Tool Path screen