When I was first testing my Gecko 540 I only connected power to the board (observing polarity of course - ie pin 12 to -48v and pin 11 to +48v), connected a wire between pin 10 and -48V on my board, and only one motor.
To power the transformer, connect the green wire to ground, the black wire to line ("L") and the white wire to neutral ("N").
16 guage wire is sufficient 14 guage is overkill and might be hard to connect.