Lets go through some logical steps please respond to each numbered question with your results. Give me as much info as you can, remember I'm troubleshooting in the blind. :-)
First the obvious:
1. Check for 5V and ground on the pics?
2. Closely look at your solder joints?
3. Did your remember to program the PIC?
4. Check to make sure your software is correctly setup?
5. Check to see if you are getting a full swing on the outputs of the opto isolators. Do that by using a parallel port toggling program to set a port pin high then low and measure the corresponding output of the opto isolator to make sure the low state is less than .7VDC in the low state and 3.5VDC or higher in the high state? Do this with the PCIStep board hooked up.
6. Does the enable (brake ) go to the enabled state?
7. Independently of the interface board do the picstep boards work manually with a simple switch to emulate enable, direction and clock?
Phil
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